S-box and T-box riboswitches and antisense RNA control a sulfur metabolic operon of Clostridium acetobutylicum
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چکیده
منابع مشابه
Antisense RNA strategies for metabolic engineering of Clostridium acetobutylicum.
We examined the effectiveness of antisense RNA (as RNA) strategies for metabolic engineering of Clostridium acetobutylicum. Strain ATCC 824(pRD4) was developed to produce a 102-nucleotide asRNA with 87% complementarity to the butyrate kinase (BK) gene. Strain ATCC 824(pRD4) exhibited 85 to 90% lower BK and acetate kinase specific activities than the control strain. Strain ATCC 824(pRD4) also ex...
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The T box and S box transcription termination control systems are widely used for control of gene expression in Gram-positive bacteria, but are rare in Gram-negative organisms. Both of these systems can be recognized in genomic data because of high conservation of primary sequence and structural elements. The T box system regulates a variety of amino acid-related genes, while the S box system i...
متن کاملDifferential expression of a Clostridium acetobutylicum antisense RNA: implications for regulation of glutamine synthetase.
The Clostridium acetobutylicum glutamine synthetase (GS) DNA region is characterized by a downstream promoter, P3, oriented toward the glnA gene, which controls the transcription of an RNA complementary to the start of the glnA mRNA. Expression of the predicted 43-base antisense RNA was demonstrated in C. acetobutylicum and Escherichia coli cells containing the cloned glnA DNA. Antisense RNA tr...
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Boolean functions and substitution boxes (S-boxes) represent the only nonlinear part in many algorithms and therefore play the crucial role in their security. Despite the fact that some algorithms today reuse theoretically secure and carefully constructed S-boxes, there is a clear need for a tool that can analyze security properties of S-boxes and hence the corresponding primitives. This need i...
متن کاملTwo-codon T-box riboswitch binding two tRNAs.
T-box riboswitches control transcription of downstream genes through the tRNA-binding formation of terminator or antiterminator structures. Previously reported T-boxes were described as single-specificity riboswitches that can bind specific tRNA anticodons through codon-anticodon interactions with the nucleotide triplet of their specifier loop (SL). However, the possibility that T-boxes might e...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 2008
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/gkn601